Kendriya Vidyalaya Sangathan written examination for PGT (BIOTECH)

Kendriya Vidyalaya Sangathan written examination for PGT (BIOTECH) KVS written examination for PGT (BIOTECH)
Kendriya Vidyalaya Sangathan written examination for PGT (BIOTECH) KVS written examination for PGT (BIOTECH)
Syllabus for written examination for PGT(BIOTECH)
Introduction to Bio-technology
Historical perspectives, scope and importance, commercial potential , interdisciplinary challenge, a quantative approach-scale up – stages in commercialization of product and process, the fermenter , aseptic operation. Manufacturing quality control, good manufacturing practices, good laboratory practices, product safety, bio safety principles-environment and health risk , assessment, bio safety regulatory guidelines and controlling agency, environmental law for hazardous drugs , microbes and GMO’S , Biotechnology related issues of Public concern, Bioethics. Marketing, Biotechnology in India and global trends.Fundamentals of Biochemical engineering-Concept of pH , buffer , physical variables , dimensions and units , measurement conventions , physical and chemical properties, data , stoichiometry , errors in data and calculation, absolute and relative un certainty and types of error statistical analysis presentation of experimental data , data analysis , trends , testing mathematical models, goodness to fit , use of graph paper with logarithmic coordination and plotting of data process flow diagrams, material balance, fluid flow and mixing , mass transfer , heat transfer, unit operations, homogenous reactions , microbial growth , substrates utilization and product formation kinetics, reactor engineering – rheology of fermentation fluids , scale up concepts, design of fermenting media, aseptic transfer, various microbial and enzyme reactors, instrumentation in bio reactors. Biotechnology and Society-Public perception of Biotechnology intellectual property, patents, reading a patent, International scenario, National scenario, Varietals protection, ethical issues in agriculture and health care.BiochemistryBiomolecules- Structure and Dynamics; Thermodynamics: concept of free energy,entropyBuilding blocks of carbohydrates – sugars and their derivatives , chemical properties of sugar , polysaccharides – glycogen , cellulose, chitin etc.Building blocks of proteins – Amino acids, Chemical properties of amino acids, regulation of amino acid metabolism and inborn errors of metabolism determination of sequencing of amnino acids , fragmentation of polypeptide chain , 3D structure of proteins, secondary , tertiary and quarternary structure of proteins, vitamins and enzymes. Lipids –simple fatty acids , Sphingosine, Glycerol and cholesterol and their chemical properties , lipid metabolism and its regulation. Nucleic acids- Nucleo tides, chemical properties , optical activities and stereo chemistry of bio molecules, polarimetry , conformations and configuration, RNA, DNA, 3D model of DNA , chromosome structure , circular and super coiled DNA. Biochemical transformations-carbohydrates metabolism-glycolytic path way, krebs cycle, homo fermentative path way. KH , PPP, photosynthesis- light reaction Kelvin cycle, nitrogen fixtation , nitrogen cycle, nitrogenase , gluconeogenesis, electron transport and oxidative phosphorylation , precursor-product relationship, supramolecular assembly, biomolecular database, biomembranes, structure and function of liposomes and their applicationsTechniques, Instrumentation and principles
Techniques based on molecular weight or size- Centrifugation and ultra centrifugation, gel permeation , osmotic potential.Techniques based on polarity- Ion exchange chromatography, elctrophoresis, isoelectric focusing , hydro phobic interaction , partition chromatography.Techniques based on spectroscopy- Colorimetry, UV visible , spectro photometry, fluorescence , spectroscopy , x-ray crystallography , mass spectrometry , radio isotopes techniques; Techniques based on solubility – Salt perceipitation , precipitation with organic solvent.Cellular techniques- Microscopy-LM,TEM,SEM cell sorting , cell fractionation , cell growth determination , electronic particle counter, culture based counting methods Genetical techniques- Chromosomal techniques- Staining , bending, pattern, Karyotyping, chromosomal painting. Mutagenic techniques- Bacterial and seed mutagenesis , recombination in bacteria, conjugation, transduction, breeding methods in plants, pedigree analysis, DNA isolation.
Cell Biology
Cell structure and components- Cell membrane – composition, Structure, membrane , associated receptors, artificial membrane, membrane proteins, principals of membrane organization, cell junction, membrane lipids. Cell organellels – Golgi bodies, Endoplasmic reticulum, lysosomes, per oxisomes, ribosomes, internatilsation of macro molecules , endo and exocytosis , mitochondrial structure and oxidative phosphorylation. Cytoskeleton- Micro tubules , micro filaments, lattice and cytosol ;Nucleus –nuclear envelope , nucleolus , chromosome tissue and organs , evolution and population , speciation , biodiversity , adaptation, natural selection, organization of life, size and complexity , interaction with environment .Cell growth and development – Cell division, cell cycles, cell communication and signal transduction , movement, nutrition, gaseous exchange , internal transport, maintaining the internal environment, reproduction, animal and plant development, immune response, apoposis, plant-pathogen relation, secondary metabolism, defence strategy in microbes and insects.